To isolate endophytic bacteria from roots, stems, and leaves of selected medicinal plants

To screen the isolated endophytic bacteria for melatonin production

To characterize melatonin-producing bacterial isolates using biochemical and molecular methods

To assess the potential role of these endophytes in plant growth promotion and stress tolerance

To explore the applicability of melatonin-producing endophytes in agricultural and biotechnological interventions

Selection of Medicinal Plants: Healthy medicinal plants will be selected based on ethnobotanical importance.

Surface Sterilization: Plant tissues (roots, stems, leaves) will be surface-sterilized to eliminate epiphytic microorganisms.

Isolation of Endophytic Bacteria: Sterilized tissues will be cultured on appropriate growth media using standard microbiological techniques.

Screening for Melatonin Production: Isolates will be screened using biochemical assays and analytical techniques such as chromatography-based methods.

Characterization of Isolates: Selected melatonin-producing strains will be characterized through morphological, biochemical, and molecular analyses.

Data Analysis: Comparative analysis will be performed to correlate melatonin production with bacterial diversity and plant source.